MM-0146-P

Calcineurin Mouse Monoclonal Antibody (VA1)

Application :

ELISA, ICC, IHC, IP, WB

Reactivity :

Bovine, rabbit, Rat

Host :

Mouse

Clonality :

Monoclonal

USD $345.00

/ 100µg

Overview

Target

Calcineurin

Target background

Within the last 30 years of research the biological roles of calcineurin have progressed from a putative inhibitor of the calmodulin dependent phosphodiesterase to the ground-breaking discovery that it is the target of immunosuppressant drugs such as cyclosporin A. As a serine/threonine protein phosphatase, calcineurin participates in a number of cellular processes and Ca2+ dependent signal transduction pathways. Calcineurin plays a key role in regulating the transcription factor NF-AT during T-cell activation, and in mediating responses of microorganisms to cation stress. Recently, this protein was studied extensively in relation to diseases such as schizophrenia and diabetes.

Target alias

Calcineurin beta-subunit

Immunogen

Full length bovine protein

Specificity

The antibody recognizes the regulatory β-subunit of calcineurin

Clone ID

VA1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: WB: 1:2000; ICC: 1:200. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Calcineurin Mouse Monoclonal Antibody (VA1)

SKU: MM-0146-P
100µg

Overview

Target

Calcineurin

Target background

Within the last 30 years of research the biological roles of calcineurin have progressed from a putative inhibitor of the calmodulin dependent phosphodiesterase to the ground-breaking discovery that it is the target of immunosuppressant drugs such as cyclosporin A. As a serine/threonine protein phosphatase, calcineurin participates in a number of cellular processes and Ca2+ dependent signal transduction pathways. Calcineurin plays a key role in regulating the transcription factor NF-AT during T-cell activation, and in mediating responses of microorganisms to cation stress. Recently, this protein was studied extensively in relation to diseases such as schizophrenia and diabetes.

Target alias

Calcineurin beta-subunit

Immunogen

Full length bovine protein

Specificity

The antibody recognizes the regulatory β-subunit of calcineurin

Clone ID

VA1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: WB: 1:2000; ICC: 1:200. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.