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Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody

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Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody


USD 355.00 /200 µL

Host: Rabbit
Reactivity: Human, Mouse, Rat
Application: WB, IF



Clathrin Light Chain (CLCa / CLCb)
Target background: 
Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.
Target alias: 
Clathrin light chain A, Lca
peptide in N term
Specificity: The antibody recognizes the N-terminal portion of Clathrin Light Chain
Clone ID: 
Lyophilized immunogen affinity purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 200 µL: IF 1:100 to 1:500; WB 1:1000-1:2000. Optimal dilution has to be determined by the user.
Research Use Only
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.