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CRB3 Rat Monoclonal Antibody (14F9)

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  • CRB3 Rat Monoclonal Antibody (14F9)
  • LEFT: Western blot analysis shows that antibody recognize the full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra) (anti-CRB3 (14F9) was used at 1:1000).  RIGHT: Western blot analysis shows that antibody recognize overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). (anti-CRB3 (14F9) was used at 1:1000). LEFT: Western blot analysis shows that antibody recognize the full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra) (anti-CRB3 (14F9) was used at 1:1000). RIGHT: Western blot analysis shows that antibody recognize overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). (anti-CRB3 (14F9) was used at 1:1000).
  • TOP: Immunofluorescence analysis of overexpressed human CRB3 in lysates of HeLa cells. BOTTOM: Immunofluorescence analysis of endogenous CRB3 expression in the mouse intestine. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine. TOP: Immunofluorescence analysis of overexpressed human CRB3 in lysates of HeLa cells. BOTTOM: Immunofluorescence analysis of endogenous CRB3 expression in the mouse intestine. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine.
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CRB3 Rat Monoclonal Antibody (14F9)

SKU:
MM-0230-P

USD 405.00 /100 µg

Clonality: 
Monoclonal
Host: Rat
Reactivity: Human, Mouse
Application: ELISA, WB, IHC, IF

QTY:

OVERVIEW

Target: 
CRB3
Target background: 
CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.
Target alias: 
Crumbs homologue 3
Immunogen: 
Full length protein
Specificity: The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.
Clone ID: 
14F9
Isotype: 
IgG2c
Preservative: 
None
Format: 
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 100 µl: WB 1:1000, IHC 1:100, IF 1:100. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.