Target:
Dopamine Beta Hydroxylase
Target background:
Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions. DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.
Target alias:
Dopamine beta-monooxygenase Dopamine Beta Hydroxylase, Dopamine-hydroxylase, DBH, oxygenase, anti-Dopamine Beta Hydroxylase, anti-Dopamine-hydroxylase, anti-DBH, oxygenase
Recommend starting dilution:
If reconstituted with deionized water in 2 ml: IHC and ICC: 1:30 - 1:50. Optimal dilution has to be determined by the user.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
