MM-0008-P

Dopamine Beta Hydroxylase (DBH) Mouse Monoclonal Antibody (DBH41)

Application :

ELISA, ICC, IHC, WB

Reactivity :

Rat

Host :

Mouse

Clonality :

Monoclonal

USD $380.00

/ 100 µL

Overview

Target

Dopamine Beta Hydroxylase (DBH)

Target background

Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions. DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.

Target alias

Dopamine beta-monooxygenase Dopamine Beta Hydroxylase, Dopamine-hydroxylase, DBH, oxygenase, anti-Dopamine Beta Hydroxylase, anti-Dopamine-hydroxylase, anti-DBH, oxygenase

Immunogen

Rat DBH

Specificity

This antibody recognizes This antibody specifically recognizes two bands of molecular weight 70 and 75 kDa, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present. DBH antibody specifically stains DBH-containing neurons and fibers in the rat central nervous system (CNS). It exhibits no cross-reactivity with either mouse, human, rabbit, guinea pig, cat or bovine DBH

Clone ID

DBH41

Isotype

IgG1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: IHC / ICC 1:1000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Dopamine Beta Hydroxylase (DBH) Mouse Monoclonal Antibody (DBH41)

SKU: MM-0008-P
100 µL

Overview

Target

Dopamine Beta Hydroxylase (DBH)

Target background

Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions. DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.

Target alias

Dopamine beta-monooxygenase Dopamine Beta Hydroxylase, Dopamine-hydroxylase, DBH, oxygenase, anti-Dopamine Beta Hydroxylase, anti-Dopamine-hydroxylase, anti-DBH, oxygenase

Immunogen

Rat DBH

Specificity

This antibody recognizes This antibody specifically recognizes two bands of molecular weight 70 and 75 kDa, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present. DBH antibody specifically stains DBH-containing neurons and fibers in the rat central nervous system (CNS). It exhibits no cross-reactivity with either mouse, human, rabbit, guinea pig, cat or bovine DBH

Clone ID

DBH41

Isotype

IgG1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: IHC / ICC 1:1000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.