Endoglycan is a CD34-related sialomucin protein highly expressed in the brain. It is also expressed on hematopoietic precursors, endothelial cells, smooth muscle cells, leukocyte subpopulations and is weakly expressed in liver. Endoglycan can act in selectin-dependant adhesion of leukocytes to vasculature and it also can be involved in inhibition of stem cell differentiation.
Podocalyxin-like protein 2, Podxl2
Lyophilized protein free tissue culture supernatant
Recommend starting dilution:
If reconstituted with deionized water in 200 µL: FC 1:400. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.