MM-0064

Endoglycan Rat Monoclonal Antibody (F4B10)

Application :

FC

Reactivity :

Human, Mouse

Host :

Rat

Clonality :

Monoclonal

USD $275.00

/ 200 µL

Overview

Target

Endoglycan

Target background

Endoglycan is a CD34-related sialomucin protein highly expressed in the brain. It is also expressed on hematopoietic precursors, endothelial cells, smooth muscle cells, leukocyte subpopulations and is weakly expressed in liver. Endoglycan can act in selectin-dependant adhesion of leukocytes to vasculature and it also can be involved in inhibition of stem cell differentiation.

Target alias

Podocalyxin-like protein 2, Podxl2

Immunogen

Peptide

Specificity

The antibody recognizes the extracellular domain of Endoglycan protein (aa121-150)

Clone ID

F4B10

Isotype

IgM

Preservative

None

Format

Lyophilized protein free tissue culture supernatant

Recommend starting dilution

If reconstituted with deionized water in 200 µL: FC 1:400. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Endoglycan Rat Monoclonal Antibody (F4B10)

SKU: MM-0064
200 µL

Overview

Target

Endoglycan

Target background

Endoglycan is a CD34-related sialomucin protein highly expressed in the brain. It is also expressed on hematopoietic precursors, endothelial cells, smooth muscle cells, leukocyte subpopulations and is weakly expressed in liver. Endoglycan can act in selectin-dependant adhesion of leukocytes to vasculature and it also can be involved in inhibition of stem cell differentiation.

Target alias

Podocalyxin-like protein 2, Podxl2

Immunogen

Peptide

Specificity

The antibody recognizes the extracellular domain of Endoglycan protein (aa121-150)

Clone ID

F4B10

Isotype

IgM

Preservative

None

Format

Lyophilized protein free tissue culture supernatant

Recommend starting dilution

If reconstituted with deionized water in 200 µL: FC 1:400. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.