This member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases has no ligand binding domain of its own and therefore cannot bind growth factors. However, it binds tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumours. [provided by RefSeq, Jul 2008]
CD340, ERBB2, HER2, NEU, NGL
ECD of recombinant HER2, aa: Thr23-Thr652
CR - 015 (generated by CCAB)
hIgG1; also available as mIgG1, scIgG1, schmIgG2a
Lyophilized protein A purified in PBS pH7.4
Recommend starting dilution:
Reconstitute with deionized water. Optimal dilution has to be determined by the user.
Research Use Only; Manufactured by CCAB under a license using Life Technologies proprietary cell lines.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.