Epitope tags have applications in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size, the protein’s biochemical properties seem not to be affected by the tag protein. Polyhistidine-tag is also known as histidine-tag, 6xHis-tag and by the trademarked name His-tag.
The antibody was raised against HHHHHH (6x His) synthetic peptide conjugated to KLH.
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100 µl: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.