Horseradish Peroxidase (HRP)
Horseradish peroxidase (HRP) is a 40 kilodalton (kDa) glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and Immunohistochemistry. A monoclonal rat anti-peroxidase antibody is a very useful and reliable reagent in the localization of a number of rat primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100 µL: IHC: 1:50 – 1:200. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.