MM-0074

Iron Regulatory Protein 1 (IRP1) Rabbit Polyclonal Antibody

Application :

WB

Reactivity :

Human, Mouse

Host :

Rabbit

Clonality :

Polyclonal

USD $345.00

/ 50 µL

Overview

Target

Iron Regulatory Protein 1 (IRP1)

Target background

The iron regulatory proteins IRP1 and IRP2 are involved in posttranscriptional regulation of cellular iron metabolism by binding to mRNA iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. IRP-1 is a bifunctional soluble protein that functions as an IRE-binding protein or as the cytoplasmic isoform of aconitase.

Target alias

Iron-responsive element-binding proteins, IRE-BP, IRBP, IRP, IFR

Immunogen

Full length protein

Specificity

The antibody recognizes the gull length IRP1 protein

Clone ID

---

Preservative

None

Format

Lyophilized serum

Recommend starting dilution

If reconstituted with deionized water in 50 µL: WB 1:2000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Iron Regulatory Protein 1 (IRP1) Rabbit Polyclonal Antibody

SKU: MM-0074
50 µL

Overview

Target

Iron Regulatory Protein 1 (IRP1)

Target background

The iron regulatory proteins IRP1 and IRP2 are involved in posttranscriptional regulation of cellular iron metabolism by binding to mRNA iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. IRP-1 is a bifunctional soluble protein that functions as an IRE-binding protein or as the cytoplasmic isoform of aconitase.

Target alias

Iron-responsive element-binding proteins, IRE-BP, IRBP, IRP, IFR

Immunogen

Full length protein

Specificity

The antibody recognizes the gull length IRP1 protein

Clone ID

---

Preservative

None

Format

Lyophilized serum

Recommend starting dilution

If reconstituted with deionized water in 50 µL: WB 1:2000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.