Iron Regulatory Protein 1 (IRP1)

The iron regulatory proteins IRP1 and IRP2 are involved in posttranscriptional regulation of cellular iron metabolism by binding to mRNA iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. IRP-1 is a bifunctional soluble protein that functions as an IRE-binding protein or as the cytoplasmic isoform of aconitase.

Iron-responsive element-binding proteins, IRE-BP, IRBP, IRP, IFR

Full length protein

The antibody recognizes the gull length IRP1 protein

---

None

Lyophilized serum

If reconstituted with deionized water in 50 µL: WB 1:2000. Optimal dilution has to be determined by the user.

Research Use Only

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.