Small G-protein dissociation stimulator (Smg-GDS) is a guanine nucleotide exchange factor which displays a wide variety of target proteins both from the Ras and Rho families. Small GTPases are known for their ability to bind GTP and hydrolyze the bound GTP to GDP. SmgGDS has been shown to be upregulated in several types of cancer such as non-small cell lung carcinoma and prostate cancer, where it promotes both proliferation and migration.
Rap1 GTPase-GDP dissociation stimulator 1, Exchange factor smgGDS, SMG GDS protein, SMG P21 stimulatory GDP/GTP exchange protein
Recommend starting dilution:
If reconstituted with deionized water in 100 µl: WB 1:1000 – 1:3000. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.