MM-0020-P

RPTPsigma Mouse Monoclonal Antibody (17G7.2)

Application :

IHC, IP, WB

Reactivity :

Human, Monkey, Mouse

Host :

Mouse

Clonality :

Monoclonal

USD $475.00

/ 100 µg

Overview

Target

RPTPsigma

Target background

Type IIa receptor protein tyrosine phosphatases (RPTPs) are a group of well-characterized proteins that are involved in axon growth and guidance during neural development. Members of this subfamily, RPTPσ, RPTPδ and LAR contain two cytoplasmic phosphatase domains and extracellular immunoglobulin domains followed by fibronectin type III repeats. RPTPσ is a type I transmembrane protein that has an apparent molecular weight of approximately 80 kDa. It is highly expressed by neurons in developing and adult mammalian nervous system. Knock-out mice for RPTPσ show growth retardation, delayed peripheral nerve development and high mortality rates. The role of RPTPσ in axon guidance during development suggests it could influence axon regeneration after injury in the adult mouse. It has been shown that RPTPσ slows axon regeneration in the adult injured CNS.

Target alias

R-PTP-S, PTPNU-3, Receptor-type tyrosine-protein phosphatase sigma, R-PTP-sigma, Ptprs

Immunogen

recombinant protein corresponding to the intracellular domain of mouse RPTP .

Specificity

The antibody recognizes the intracellular sub-unit of RPTPσ.

Clone ID

17G7.2

Isotype

IgG1 kappa

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: WB 1:2000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

RPTPsigma Mouse Monoclonal Antibody (17G7.2)

SKU: MM-0020-P
100 µg

Overview

Target

RPTPsigma

Target background

Type IIa receptor protein tyrosine phosphatases (RPTPs) are a group of well-characterized proteins that are involved in axon growth and guidance during neural development. Members of this subfamily, RPTPσ, RPTPδ and LAR contain two cytoplasmic phosphatase domains and extracellular immunoglobulin domains followed by fibronectin type III repeats. RPTPσ is a type I transmembrane protein that has an apparent molecular weight of approximately 80 kDa. It is highly expressed by neurons in developing and adult mammalian nervous system. Knock-out mice for RPTPσ show growth retardation, delayed peripheral nerve development and high mortality rates. The role of RPTPσ in axon guidance during development suggests it could influence axon regeneration after injury in the adult mouse. It has been shown that RPTPσ slows axon regeneration in the adult injured CNS.

Target alias

R-PTP-S, PTPNU-3, Receptor-type tyrosine-protein phosphatase sigma, R-PTP-sigma, Ptprs

Immunogen

recombinant protein corresponding to the intracellular domain of mouse RPTP .

Specificity

The antibody recognizes the intracellular sub-unit of RPTPσ.

Clone ID

17G7.2

Isotype

IgG1 kappa

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: WB 1:2000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.