1019

Serotonin Rat Monoclonal Antibody (YC5/45)

Application :

A, IF, IHC

Host :

Rat

Clonality :

Monoclonal

USD $390.00

/ 0.5 mL

Overview

Target

Serotonin

Target background

Serotonin or 5-hydroxytryptamine is a monoamine neurotransmitter. It’s synthesized in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Because these neurons profile diffusely throughout the brain, serotonin can affect various brain functions. It also interacts with many other neurotransmitters, either directly through neurons that use both serotonin and another neurotransmitter, or by serotonin neurons influencing neurons that primarily use these other transmitters.

Target alias

5 hydroxytryptamine, 5HT, 25689, 5 HT5A, 5 HT, 5HT 15563, 5HT15563, 5 HT 5A

Immunogen

Serotonin (C10H12N2O)

Specificity

Formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in haemagglutination tests. The antibody reacts with serotonin in neurons and recognises serotonergic sites in fixed tissue sections. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. By immunocytochemistry no cross-reactivity with dopamine and noradrenaline (6). By red cell agglutination, no cross-reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine, melatonin

Clone ID

YC5/45

Isotype

IgG

Preservative

0.05% thimerosal (Thimerosal free version (MM-1019) ask for a quote)

Format

Lyophilized tissue culture supernatant

Recommend starting dilution

If reconstituted with deionized water in 0.5 mL: IHC 1:100 - 1:200 for formaldehyde fixed tissue. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Serotonin Rat Monoclonal Antibody (YC5/45)

SKU: 1019
0.5 mL

Overview

Target

Serotonin

Target background

Serotonin or 5-hydroxytryptamine is a monoamine neurotransmitter. It’s synthesized in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Because these neurons profile diffusely throughout the brain, serotonin can affect various brain functions. It also interacts with many other neurotransmitters, either directly through neurons that use both serotonin and another neurotransmitter, or by serotonin neurons influencing neurons that primarily use these other transmitters.

Target alias

5 hydroxytryptamine, 5HT, 25689, 5 HT5A, 5 HT, 5HT 15563, 5HT15563, 5 HT 5A

Immunogen

Serotonin (C10H12N2O)

Specificity

Formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in haemagglutination tests. The antibody reacts with serotonin in neurons and recognises serotonergic sites in fixed tissue sections. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. By immunocytochemistry no cross-reactivity with dopamine and noradrenaline (6). By red cell agglutination, no cross-reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine, melatonin

Clone ID

YC5/45

Isotype

IgG

Preservative

0.05% thimerosal (Thimerosal free version (MM-1019) ask for a quote)

Format

Lyophilized tissue culture supernatant

Recommend starting dilution

If reconstituted with deionized water in 0.5 mL: IHC 1:100 - 1:200 for formaldehyde fixed tissue. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.