Sic1 participates in cell-cycle regulation of budding yeast, Saccharomyces cerevisiae. The protein acts as a stoichiometric inhibitor that binds to B-type cyclin-Cdk1 complexes, which, through precise temporal activation and inactivation, drive progression through the cell cycle. Multi-site phosphorylation of Sic1 leading to ubiquitination and destruction of the protein activates the Clb5,6-Cdk1 complex and promotes entry into from the G1 (Gap1) into the S (Synthesis) phase of the cell cycle, during which DNA is replicated. Mutants of Sic1 demonstrate a delay shifting from anaphase to telophase.
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100 µl: WB: 1:200. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.