Target background:
Somatostatin or growth hormone-inhibiting hormone (GHIH) is a peptide hormone that inhibits the release of somatotropin (growth hormone) from the anterior pituitary gland in the hypothalamus. Somatostatin also inhibits the release of peptide hormones in many other tissues by binding to high-affinity G protein-coupled somatostatin receptors. Two active forms have been described: a form that is 14 amino acids in length and a form that is 28 amino acids in length, which are produced by alternate cleavage of the single pre-pro-protein encoded by this gene.
Target alias:
Growth hormone release-inhibiting factor
Immunogen:
Synthetic peptide corresponding to amino acids 1-14 of cyclic somatostatin
Specificity: The antibody recognizes somatostatin. This antibody does not cross-react with a number of other neuropeptides (e.g. enkephalins, other endorphins, substance P, CGRP) and partially cross-reacts with somatostatin fragments.
Recommend starting dilution:
If reconstituted with deionized water in 1 mL: 1:50 - 1:200 for formaldehyde fixed tissue. Optimal dilution has to be determined by the user.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
