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Substance P / HRP Bispecific Monoclonal Antibody (P4C1)

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Substance P / HRP Bispecific Monoclonal Antibody (P4C1)


USD 355.00 /2 mL

Chimeric Monoclonal
Host: Rat, Mouse
Reactivity: Human, predicted all mammals, crab, pigeons
Application: IHC, ELISA



Substance P
Target background: 
This antibody is a bispecific monoclonal antibody capable of simultaneously recognize the horseradish peroxidase (HRP) and Substance P. Bi-specific monoclonal antibodies have been successfully used in one step immunocytochemistry and immuno-dot assays. These results demonstrated that bispecific antibodies can be applied in quick and simple staining procedures and provide intense immunostaining. The P4C1 antibody has been successfully used in immunoassays and immunohistochemistry. Light and electron micrographs of rat tissue developed using this antibody clearly demonstrate substance P immunoreactive sites with very minimal non-specific background binding.
Target alias: 
Substance P, tachykinin, neuropeptide, neurokinin, SP, anti-tachykinin, anti-neuropeptide, anti-neurokinin, anti-SP
Substance P and Full Lenght HRP
Specificity: This is a bispecific antibody which recognizes (A) Substance P and (B) Horseradish peroxidase types I, II, VI, IX, X. This antibody recognizes the COOH-terminal end of substance P. It does not recognize Leu- or Met enkephalin, somatostatin or beta-endorphin; cross-reactivity with eledoisin: 5%. It recognizes Substance P in post-mortem tissue of the human brain.
Clone ID: 
IgG2a / IgG1
0.05% thimerosal
Lyophilized tissue culture supernatant
Recommend starting dilution: 
If reconstituted with deionized water in 2 ml: IHC: 1:100 - 1:200. Optimal dilution has to be determined by the user.
Research Use Only
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.