Additional information
| Weight | 0.03 lbs |
|---|---|
| Dimensions | 0.3 × 0.25 × 0.1 in |
MM-0029-P
Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)
Application :
ChIP, IF, IHC, WBReactivity :
Arabidopsis, Drosophila, Human, MouseHost :
MouseClonality :
MonoclonalOverview
Target
Ubiquitinated H2B
Target background
It has been demonstrated that histone modifications are implicated in the regulation of transcription. Recent studies have revealed complex links between H2B ubiquitination and the control of transcription. Some studies suggest that H2B ubiquitination plays a role in transcriptional silencing whereas others suggest a role in transcriptional initiation and elongation. This antibody was raised against a branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B.
Target alias
Ubiquitinated Histone H2B, Ubiquitin Histone H2B, H2B, ub histone h2b, ub h2b
Immunogen
Branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B
Specificity
The antibody recognizes the K120 ubiquitination of the human histone H2B with no detectable cross-reactivity with either non-ubiquitinated H2B or ubiquitinated histone H2A.
Clone ID
NRO3
Isotype
IgG2a
Preservative
None
Format
Purified with protein G, stored in PBS pH 7.4 and lyophilized.
Recommend starting dilution
If reconstituted with deionized water in 50 µL: WB 2-3 µg/ml; ChIP 3-5 µg per sample; IHC: 1:200 – 1:500. Optimal dilution has to be determined by the user.
Limitations
Research Use Only
Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
References
Product datasheet
Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)
SKU: MM-0029-P
100 µg
Overview
Target
Ubiquitinated H2B
Target background
It has been demonstrated that histone modifications are implicated in the regulation of transcription. Recent studies have revealed complex links between H2B ubiquitination and the control of transcription. Some studies suggest that H2B ubiquitination plays a role in transcriptional silencing whereas others suggest a role in transcriptional initiation and elongation. This antibody was raised against a branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B.
Target alias
Ubiquitinated Histone H2B, Ubiquitin Histone H2B, H2B, ub histone h2b, ub h2b
Immunogen
Branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B
Specificity
The antibody recognizes the K120 ubiquitination of the human histone H2B with no detectable cross-reactivity with either non-ubiquitinated H2B or ubiquitinated histone H2A.
Clone ID
NRO3
Isotype
IgG2a
Preservative
None
Format
Purified with protein G, stored in PBS pH 7.4 and lyophilized.
Recommend starting dilution
If reconstituted with deionized water in 50 µL: WB 2-3 µg/ml; ChIP 3-5 µg per sample; IHC: 1:200 – 1:500. Optimal dilution has to be determined by the user.
Limitations
Research Use Only
Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.