MM-0143

Alpha-Tubulin cleaved by caspase-6 Rabbit Polyclonal Antibody

Application :

IHC, WB

Reactivity :

Bovine, Human, Mouse

Host :

Rabbit

Clonality :

Polyclonal

USD $345.00

/ 50 µL

Overview

Target

Tubulin cleaved by caspase-6

Target background

Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of α- and β-tubulin. α-Tubulin cleaved by caspase-6 can be found in neurons, neurofibrillary tangles, neutrophil threads, and neuritic plaques in Alzheimer disease. α-Tubulin has been found to associate with the axonal microtubule-associated protein Tau, indicating that the cleavage of the functional α-Tubulin C-terminal domain by caspase-6 could alter the state of microtubules in neurons.

Target alias

α-Tubulin cleaved by casp 6

Immunogen

EEVGVD peptide

Specificity

The antibody recognizes the sequence (EEVGVD) in tubulin protein. It is specific for Caspase 6 cleaved α-Tubulin. The antibody was tested in IHC and western blots of samples with Alzheimer disease (AD) (Bovine brain sample, human samples, cells lines, mice hippocampus and whole brains). When tested in null mice with IHC and WB, no immunoreactivity was observed.

Clone ID

---

Preservative

None

Format

Lyophilized serum

Recommend starting dilution

If reconstituted with deionized water in 50 µl: WB 1:1000; IHC 1:1000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Alpha-Tubulin cleaved by caspase-6 Rabbit Polyclonal Antibody

SKU: MM-0143
50 µL

Overview

Target

Tubulin cleaved by caspase-6

Target background

Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of α- and β-tubulin. α-Tubulin cleaved by caspase-6 can be found in neurons, neurofibrillary tangles, neutrophil threads, and neuritic plaques in Alzheimer disease. α-Tubulin has been found to associate with the axonal microtubule-associated protein Tau, indicating that the cleavage of the functional α-Tubulin C-terminal domain by caspase-6 could alter the state of microtubules in neurons.

Target alias

α-Tubulin cleaved by casp 6

Immunogen

EEVGVD peptide

Specificity

The antibody recognizes the sequence (EEVGVD) in tubulin protein. It is specific for Caspase 6 cleaved α-Tubulin. The antibody was tested in IHC and western blots of samples with Alzheimer disease (AD) (Bovine brain sample, human samples, cells lines, mice hippocampus and whole brains). When tested in null mice with IHC and WB, no immunoreactivity was observed.

Clone ID

---

Preservative

None

Format

Lyophilized serum

Recommend starting dilution

If reconstituted with deionized water in 50 µl: WB 1:1000; IHC 1:1000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.