MM-0142

Amyloid precursor protein cleaved by caspase-6 Rabbit Polyclonal Antibody

Application :

WB

Reactivity :

Human

Host :

Rabbit

Clonality :

Polyclonal

USD $275.00

/ 50 µL

Overview

Target

Amyloid precursor protein cleaved by caspase-6

Target background

Amyloid precursor protein (APP) functions as a cell surface receptor in the synapses of neurons and is implicated in neurite growth, neuronal adhesion, axonogenesis and cell mobility. APP is known as the precursor molecule whose proteolysis generates beta amyloid. Defects in APP are the cause of Alzheimer’s disease type 1 and of amyloidosis cerebroarterial. Caspase activation has been found to have a central role in neuronal death and chronic neurodegenerations such as Huntington’s, Parkinson’s and Alzheimer’s diseases. Proteolytic cleavage of specific substrates has also been demonstrated to be an important cellular event in the pathogenesis of AD and inhibition of cleavage of APP by caspase-6 was demonstrated to suppress synapse loss, dentate gyral atrophy, and memory loss.

Immunogen

ISEVKMD peptide

Specificity

The antibody recognizes the sequence (ISEVKMD) in Amyloid precursor protein (APP).

Clone ID

---

Preservative

None

Format

Lyophilized serum

Recommend starting dilution

If reconstituted with deionized water in 50 µl: WB 1: 5000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Amyloid precursor protein cleaved by caspase-6 Rabbit Polyclonal Antibody

SKU: MM-0142
50 µL

Overview

Target

Amyloid precursor protein cleaved by caspase-6

Target background

Amyloid precursor protein (APP) functions as a cell surface receptor in the synapses of neurons and is implicated in neurite growth, neuronal adhesion, axonogenesis and cell mobility. APP is known as the precursor molecule whose proteolysis generates beta amyloid. Defects in APP are the cause of Alzheimer’s disease type 1 and of amyloidosis cerebroarterial. Caspase activation has been found to have a central role in neuronal death and chronic neurodegenerations such as Huntington’s, Parkinson’s and Alzheimer’s diseases. Proteolytic cleavage of specific substrates has also been demonstrated to be an important cellular event in the pathogenesis of AD and inhibition of cleavage of APP by caspase-6 was demonstrated to suppress synapse loss, dentate gyral atrophy, and memory loss.

Immunogen

ISEVKMD peptide

Specificity

The antibody recognizes the sequence (ISEVKMD) in Amyloid precursor protein (APP).

Clone ID

---

Preservative

None

Format

Lyophilized serum

Recommend starting dilution

If reconstituted with deionized water in 50 µl: WB 1: 5000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.