MM-0280-P

BACE-1 Mouse Monoclonal Antibody (21C5_F11)

Application :

ELISA, WB

Reactivity :

Human, Rat

Host :

Mouse

Clonality :

Monoclonal

USD $295.00

/ 100 µg

Overview

Target

BACE-1

Target background

The function of beta-secretase converting enzyme (BACE) has been identified as the initial step in the formation of harmful amyloid-beta peptides from the amyloid-beta precursor protein linked to Alzheimer’s disease. Transgenic mice with a deficiency of the enzyme BACE lack these amyloid-beta peptide formation peptides, suggesting a crucial role for BACE-1 in the neurodegeneration prevalent in Alzheimer’s patients. In order to reach its mature, functional state, the protein BACE-1 must undergo several modifications, including a multimerization step. The consequent activity of BACE-1 has been found to be regulated by two specific aspartic protease active site motifs.

Target alias

Beta-secretase 1, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Membrane-associated aspartic protease 2, Memapsin-2, Aspartyl protease 2, and ASP2

Immunogen

Synthetic peptide in the N-terminal active site of human BACE-1.

Specificity

The antibody recognizes the N-terminal active site BACE-1.

Clone ID

21C5_F11

Isotype

IgG1 kappa

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

BACE-1 Mouse Monoclonal Antibody (21C5_F11)

SKU: MM-0280-P
100 µg

Overview

Target

BACE-1

Target background

The function of beta-secretase converting enzyme (BACE) has been identified as the initial step in the formation of harmful amyloid-beta peptides from the amyloid-beta precursor protein linked to Alzheimer’s disease. Transgenic mice with a deficiency of the enzyme BACE lack these amyloid-beta peptide formation peptides, suggesting a crucial role for BACE-1 in the neurodegeneration prevalent in Alzheimer’s patients. In order to reach its mature, functional state, the protein BACE-1 must undergo several modifications, including a multimerization step. The consequent activity of BACE-1 has been found to be regulated by two specific aspartic protease active site motifs.

Target alias

Beta-secretase 1, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Membrane-associated aspartic protease 2, Memapsin-2, Aspartyl protease 2, and ASP2

Immunogen

Synthetic peptide in the N-terminal active site of human BACE-1.

Specificity

The antibody recognizes the N-terminal active site BACE-1.

Clone ID

21C5_F11

Isotype

IgG1 kappa

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.