MM-0290

Carcinoembryonic antigen (CEA) Mouse Monoclonal Antibody (C234)

Application :

ELISA, IHC

Reactivity :

Human

Host :

Mouse

Clonality :

Monoclonal

USD $295.00

/ 100 µg

Overview

Target

Carcinoembryonic antigen (CEA)

Target background

Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly typical, high-molecular weight, cell surface glycoprotein over-expressed in adenocarcinomas of entero-dermally-derived digestive system epithelia and in fetal colon. The majority of antibodies induced against CEA would cross-react with a variety of proteins present in normal tissues such as non-specific cross-reacting antigen (NCA). Highly specific anti-CEA mouse monoclonal antibody was produced as a result of a unique imunization protocol.

Target alias

CEA

Immunogen

Physically modified CEA

Specificity

This antibody is CEA specific. It does not recognize NCA, neither in enzyme linked immunosorbent assay (ELISA) not on Western blots of crude tissue extracts. This antibody binds to CEA producing human adenocarcinoma cells (e.g. LS174T,WiDr) but does not bind to CEA non-secreting human adenocarcinoma cells (Colo 320DM). It does not immunostain cryocuts of any normal human tissues tested (artery, heart muscle, kidney, large bowel, liver, lung, skeletal muscle, skin, small gut, spleen, testes or vein) neither does it react with any antigens on human blood cells when separated by fluoresceine actuated cell sorter (FACS). Immunohistochemical analysis of cancerous tissues revealed that the antibody bound selectively adenocarcinoma and not carcinoid tumor of the colon, as well as tumor- associated CEA in breast, gastric and lung neoplasms, while there was no immunohistochemical evidence of cross-reactivity with NCA. A remarkable feature of this antibody is its ability to distinguish immunohistochemically between cancer and residual normal tissue within the same organ.

Clone ID

C234

Isotype

IgG kappa

Preservative

None

Format

Lyophilized Protein A purified in PBS pH7.4 (Full IgG)

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Carcinoembryonic antigen (CEA) Mouse Monoclonal Antibody (C234)

SKU: MM-0290
100 µg

Overview

Target

Carcinoembryonic antigen (CEA)

Target background

Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly typical, high-molecular weight, cell surface glycoprotein over-expressed in adenocarcinomas of entero-dermally-derived digestive system epithelia and in fetal colon. The majority of antibodies induced against CEA would cross-react with a variety of proteins present in normal tissues such as non-specific cross-reacting antigen (NCA). Highly specific anti-CEA mouse monoclonal antibody was produced as a result of a unique imunization protocol.

Target alias

CEA

Immunogen

Physically modified CEA

Specificity

This antibody is CEA specific. It does not recognize NCA, neither in enzyme linked immunosorbent assay (ELISA) not on Western blots of crude tissue extracts. This antibody binds to CEA producing human adenocarcinoma cells (e.g. LS174T,WiDr) but does not bind to CEA non-secreting human adenocarcinoma cells (Colo 320DM). It does not immunostain cryocuts of any normal human tissues tested (artery, heart muscle, kidney, large bowel, liver, lung, skeletal muscle, skin, small gut, spleen, testes or vein) neither does it react with any antigens on human blood cells when separated by fluoresceine actuated cell sorter (FACS). Immunohistochemical analysis of cancerous tissues revealed that the antibody bound selectively adenocarcinoma and not carcinoid tumor of the colon, as well as tumor- associated CEA in breast, gastric and lung neoplasms, while there was no immunohistochemical evidence of cross-reactivity with NCA. A remarkable feature of this antibody is its ability to distinguish immunohistochemically between cancer and residual normal tissue within the same organ.

Clone ID

C234

Isotype

IgG kappa

Preservative

None

Format

Lyophilized Protein A purified in PBS pH7.4 (Full IgG)

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.