MM-0033

Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody

Application :

IF, WB

Reactivity :

Human, Mouse, Rat

Host :

Rabbit

Clonality :

Polyclonal

USD $335.00

/ 200 µL

Overview

Target

Clathrin Light Chain (CLCa / CLCb)

Target background

Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.

Target alias

Clathrin light chain A, Lca

Immunogen

peptide in N term

Specificity

The antibody recognizes the N-terminal portion of Clathrin Light Chain

Clone ID

---

Preservative

None

Format

Lyophilized immunogen affinity purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 200 µL: IF 1:100 to 1:500; WB 1:1000-1:2000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody

SKU: MM-0033
200 µL

Overview

Target

Clathrin Light Chain (CLCa / CLCb)

Target background

Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.

Target alias

Clathrin light chain A, Lca

Immunogen

peptide in N term

Specificity

The antibody recognizes the N-terminal portion of Clathrin Light Chain

Clone ID

---

Preservative

None

Format

Lyophilized immunogen affinity purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 200 µL: IF 1:100 to 1:500; WB 1:1000-1:2000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.