Dscam

Down syndrome cell adhesion molecule (Dscam) is a cell adhesion molecule that plays a role in neuronal self-avoidance. It promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Dscam mediates, within retinal amacrine and ganglion cell subtypes, both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. It is the receptor for netrin that is required for axon guidance independently of and in collaboration with the receptor DCC. In spinal cord development, it plays a role in guiding commissural axons during projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Dscam enhances netrin-induced phosphorylation of PAK1 and FYN. It mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. Dscam interacts with DCC, Netrin-1, PAK1 and RAC1.

A specific antibody against the chicken Dscam C-terminal region (amino acids 1207 to 1527) .

DS2-176

IgG1 kappa

None

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

If reconstituted with deionized water in 100 µL: IHC 1:250 to 1:500. Optimal dilution has to be determined by the user.

Research Use Only

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.