MM-0176-P

Epcam ICD Mouse Monoclonal Antibody (4A7)

Application :

ELISA, IHC, WB

Reactivity :

Human, Mouse

Host :

Mouse

Clonality :

Monoclonal

USD $303.20

/ 100 µg

Additional information

Weight 0.03 lbs
Dimensions 0.3 × 0.25 × 0.1 in

Overview

Target

Epcam ICD

Target background

Epithelial cell adhesion molecule (EpCAM) is a 40-kDa epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

Target alias

Epithelial Cell Adhesion Molecule Intracellular Domain

Specificity

The antibody recognizes the intracellular domain of human EpCAM.

Clone ID

4A7

Isotype

IgG1

Preservative

None

Format

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

Recommend starting dilution

If reconstituted with deionized water in 100 µl: WB 1:1000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Epcam ICD Mouse Monoclonal Antibody (4A7)

SKU: MM-0176-P
100 µg

Overview

Target

Epcam ICD

Target background

Epithelial cell adhesion molecule (EpCAM) is a 40-kDa epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

Target alias

Epithelial Cell Adhesion Molecule Intracellular Domain

Specificity

The antibody recognizes the intracellular domain of human EpCAM.

Clone ID

4A7

Isotype

IgG1

Preservative

None

Format

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

Recommend starting dilution

If reconstituted with deionized water in 100 µl: WB 1:1000. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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