MM-0071-P

HHV-6 Variant A IE2 Mouse Monoclonal Antibody (P6H8)

Application :

ELISA, IF, WB

Reactivity :

HHV6 variant A

Host :

Mouse

Clonality :

Monoclonal

USD $345.05

/ 100 µg

Additional information

Weight 0.03 lbs
Dimensions 0.3 × 0.25 × 0.1 in

Overview

Target

HHV-6 Variant A IE2

Target background

HHV-6 is a double stranded DNA virus belonging to the betaherpesvirinae subfamily. HHV-6 viruses comprise two distinct species: HHV-6A and HHV-6B. HHV-6 is a T-cell lymphotropic virus with high affinity for CD4 lymphocytes and is capable of infecting virtually all human beings. HHV-6B is responsible for at least 97% of HHV-6 infections in the USA while HHV-6A is frequently associated with patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6A and HHV-6B proteins share more than 92% of their amino acids composition. However, the immediate-early protein locus A, has less than 70% of homology between the two species. At this locus, IE1 and IE2 proteins are encoded with IE2 being the most divergent between HHV-6A and HHV-6B. IE2 is expressed within few hours’ post-infection and is a potent transactivator of multiple cellular and viral promoters.

Target alias

Immediate-early protein 2, U90/U87/U86, BCLF0

Immunogen

Full length IE2

Specificity

The antibody recognizes the extracellular domain (aa 1078-1089 ) of protein IE2

Clone ID

P6H8

Isotype

IgG1 kappa

Preservative

None

Format

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

Recommend starting dilution

If reconstituted with deionized water in 100 µL: IF 1:50 to 1:500. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

HHV-6 Variant A IE2 Mouse Monoclonal Antibody (P6H8)

SKU: MM-0071-P
100 µg

Overview

Target

HHV-6 Variant A IE2

Target background

HHV-6 is a double stranded DNA virus belonging to the betaherpesvirinae subfamily. HHV-6 viruses comprise two distinct species: HHV-6A and HHV-6B. HHV-6 is a T-cell lymphotropic virus with high affinity for CD4 lymphocytes and is capable of infecting virtually all human beings. HHV-6B is responsible for at least 97% of HHV-6 infections in the USA while HHV-6A is frequently associated with patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6A and HHV-6B proteins share more than 92% of their amino acids composition. However, the immediate-early protein locus A, has less than 70% of homology between the two species. At this locus, IE1 and IE2 proteins are encoded with IE2 being the most divergent between HHV-6A and HHV-6B. IE2 is expressed within few hours’ post-infection and is a potent transactivator of multiple cellular and viral promoters.

Target alias

Immediate-early protein 2, U90/U87/U86, BCLF0

Immunogen

Full length IE2

Specificity

The antibody recognizes the extracellular domain (aa 1078-1089 ) of protein IE2

Clone ID

P6H8

Isotype

IgG1 kappa

Preservative

None

Format

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

Recommend starting dilution

If reconstituted with deionized water in 100 µL: IF 1:50 to 1:500. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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