MM-0260-P

HIV-1 (gp41) Human Monoclonal Antibody (50-69)

Application :

Competition Assay, ELISA, Radioimmunoprecipitation (RIP)

Reactivity :

HIV-1

Host :

Human

Clonality :

Monoclonal

USD $485.00

/ 100 µg

Overview

Target

HIV-1 (gp41)

Target background

Of the two types of Human immunodeficiency virus (HIV) which have been characterized, HIV-1 is more infective and is prevalent globally. The HIV virion is encapsulated by a viral envelope that contains complexes of surface proteins, which are important for the virus to attach and fuse to infect target cells. A precursor molecule known as gp160 (glycoprotein 160) is cleaved by proteases into gp120 (glycoprotein 120) and gp41 (glycoprotein 41), glycoproteins that make up the envelope proteins of HIV. Glycoprotein gp41 is embedded in the viral membrane and can interact with other gp41 proteins. The proteins gp41 and gp120 form a complex which is thought to undergo conformational changes in order to initiate membrane fusion of the virus with a cell.

Immunogen

The antibody was generated from HIV-infected individuals by a method based on the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) followed by fusion with heteromyeloma cells.

Specificity

The antibody recognizes the HIV-1 protein gp41. (amino acids 579-613)

Clone ID

50-69

Isotype

IgG1 kappa

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

References

Product datasheet

HIV-1 (gp41) Human Monoclonal Antibody (50-69)

SKU: MM-0260-P
100 µg

Overview

Target

HIV-1 (gp41)

Target background

Of the two types of Human immunodeficiency virus (HIV) which have been characterized, HIV-1 is more infective and is prevalent globally. The HIV virion is encapsulated by a viral envelope that contains complexes of surface proteins, which are important for the virus to attach and fuse to infect target cells. A precursor molecule known as gp160 (glycoprotein 160) is cleaved by proteases into gp120 (glycoprotein 120) and gp41 (glycoprotein 41), glycoproteins that make up the envelope proteins of HIV. Glycoprotein gp41 is embedded in the viral membrane and can interact with other gp41 proteins. The proteins gp41 and gp120 form a complex which is thought to undergo conformational changes in order to initiate membrane fusion of the virus with a cell.

Immunogen

The antibody was generated from HIV-infected individuals by a method based on the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) followed by fusion with heteromyeloma cells.

Specificity

The antibody recognizes the HIV-1 protein gp41. (amino acids 579-613)

Clone ID

50-69

Isotype

IgG1 kappa

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

References