HIV-1 (p24)

Of the two types of Human immunodeficiency virus (HIV) which have been characterized, HIV-1 is more infective and is prevalent globally. The protein known as p24 is a 24 kDa protein often referred to as the core or capsid protein of HIV-1. Levels of p24 in blood may be used as indicators of the progression of HIV. The HIV virion is encapsulated by a viral envelope that contains complexes of surface proteins including glycoprotein 120 (gp120), which are important for the virus to attach and fuse to infect target cells. This antibody was generated from HIV-infected individuals by the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) method.

This antibody was generated from HIV-infected individuals by a method based on the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) followed by fusion with heteromyeloma cells.

The antibody recognizes the p24 core or capsid protein of HIV-1. Specific for: HIV-1

28B7

IgG1 lambda

None

Purified with protein G, stored in PBS pH 7.4 and lyophilized. (Unlabeled)

Reconstitute with 100 µL of deionized water (1 µg/µL), use a 1:5000- 1:7500 dilution for flow cytometry. Optimal dilution has to be determined by the user.

Research Use Only

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.