MM-0012-P

Horseradish Peroxidase (HRP) Monoclonal Antibody (MAP A6-2)

Application :

IHC

Reactivity :

Horseradish

Host :

Mouse

Clonality :

Monoclonal

USD $380.00

/ 100 µg

Overview

Target

Horseradish Peroxidase (HRP)

Target background

Horseradish peroxidase (HRP) is a 40kDa glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and immunohistochemistry. A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.

Immunogen

HRP protein

Specificity

A sensitive antibody against horseradish peroxidase (HRP). This antibody was raised against the full horseradish peroxidase (HRP) protein

Clone ID

MAP A6-2

Isotype

IgG1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: IHC 1:30 - 1:60. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Horseradish Peroxidase (HRP) Monoclonal Antibody (MAP A6-2)

SKU: MM-0012-P
100 µg

Overview

Target

Horseradish Peroxidase (HRP)

Target background

Horseradish peroxidase (HRP) is a 40kDa glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and immunohistochemistry. A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.

Immunogen

HRP protein

Specificity

A sensitive antibody against horseradish peroxidase (HRP). This antibody was raised against the full horseradish peroxidase (HRP) protein

Clone ID

MAP A6-2

Isotype

IgG1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: IHC 1:30 - 1:60. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.