MM-0199-P

Human ICAM-3 Mouse Monoclonal Antibody (MA4)

Application :

FC, WB

Reactivity :

Human

Host :

Mouse

Clonality :

Monoclonal

USD $255.00

/ 100 µg

Additional information

Weight 0.03 lbs
Dimensions 0.3 × 0.25 × 0.1 in

Overview

Target

Human ICAM-3

Target background

Rapid clearing of apoptotic cells by phagocytes may prevent tissue damage. Studies show that ICAM-3 (also known as CD50), expressed on leukocytes, interacts with macrophages during apoptotic cell clearing (Torr et al., 2012). ICAM-3 plays an important role in the recruitment and tethering of macrophages during the process of clearing apoptotic human leukocytes, and ICAM-3 deficient cells have reduced clearance of apoptotic cells by phagocytes. This antibody, (MA4), has been shown to block the interaction of apoptotic leukocytes with macrophages/phagocytes.

Target alias

CD50, Intercellular adhesion molecule 3

Immunogen

Mammalian recombinant protein

Specificity

The antibody recognizes the domains 1-2 of intercellular adhesion molecule (ICAM)-3. This antibody has been shown to block the interaction of apoptotic leukocytes with macrophages/phagocytes.

Clone ID

MA4

Isotype

IgG1 kappa

Preservative

None

Format

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Human ICAM-3 Mouse Monoclonal Antibody (MA4)

SKU: MM-0199-P
100 µg

Overview

Target

Human ICAM-3

Target background

Rapid clearing of apoptotic cells by phagocytes may prevent tissue damage. Studies show that ICAM-3 (also known as CD50), expressed on leukocytes, interacts with macrophages during apoptotic cell clearing (Torr et al., 2012). ICAM-3 plays an important role in the recruitment and tethering of macrophages during the process of clearing apoptotic human leukocytes, and ICAM-3 deficient cells have reduced clearance of apoptotic cells by phagocytes. This antibody, (MA4), has been shown to block the interaction of apoptotic leukocytes with macrophages/phagocytes.

Target alias

CD50, Intercellular adhesion molecule 3

Immunogen

Mammalian recombinant protein

Specificity

The antibody recognizes the domains 1-2 of intercellular adhesion molecule (ICAM)-3. This antibody has been shown to block the interaction of apoptotic leukocytes with macrophages/phagocytes.

Clone ID

MA4

Isotype

IgG1 kappa

Preservative

None

Format

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

Recommend starting dilution

Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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