Antibodies diluted in glycerol can be kept at 4ºC for up to 2 months and should be kept at -20ºC for long-term storage (1 year). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. After the maximum long-term storage period (1 year -20ºC or 2 months 4ºC) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.