MM-0017-P

Leishmania A2 Mouse Monoclonal Antibody (C9)

Application :

IF, IP, WB

Reactivity :

Leishmania donovani, Leishmania infantum

Host :

Mouse

Clonality :

Monoclonal

USD $345.00

/ 100 µg

Overview

Target

Leishmania A2

Target background

Leishmania donovani is a protozoa parasite responsible for human visceral leishmaniasis. During their life cycle the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non-flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote-specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.

Immunogen

The anti-A2 antibody recognizes only the amastigote form of the A2 protein. The anti-A2 antibody recognizes the sequence QSVGPLSVGP from the parasite, Leishmania donovani.

Specificity

A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. It is specific to A2 protein from Leishmania donovani and in a highly probable manner to other species which expresses A2. It won’t be detected in species where the A2 is a non-expressed pseudogenes such as Leishmania major.

Clone ID

C9

Isotype

IgG1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: WB 1:100. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Product datasheet

Leishmania A2 Mouse Monoclonal Antibody (C9)

SKU: MM-0017-P
100 µg

Overview

Target

Leishmania A2

Target background

Leishmania donovani is a protozoa parasite responsible for human visceral leishmaniasis. During their life cycle the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non-flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote-specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.

Immunogen

The anti-A2 antibody recognizes only the amastigote form of the A2 protein. The anti-A2 antibody recognizes the sequence QSVGPLSVGP from the parasite, Leishmania donovani.

Specificity

A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. It is specific to A2 protein from Leishmania donovani and in a highly probable manner to other species which expresses A2. It won’t be detected in species where the A2 is a non-expressed pseudogenes such as Leishmania major.

Clone ID

C9

Isotype

IgG1

Preservative

None

Format

Lyophilized protein G purified in PBS pH7.4

Recommend starting dilution

If reconstituted with deionized water in 100 µL: WB 1:100. Optimal dilution has to be determined by the user.

Limitations

Research Use Only

Storage

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.