Pseudomonas aeruginosa Inner Core

Pseudomonas aeruginosa is a Gram-negative bacterium widely distributed in nature and causing opportunistic infections in humans. P. aeruginosa is an important bacterial pathogen of nosocomial (hospital derived) infections, and it can also cause life threatening diseases in patients with cancer, burn wounds, cystic fibrosis and those that have received immunosuppressive therapy. Lipopolysaccharide (LPS) is an integral component of the P. aeruginosa cell envelope, occupying the outer leaflet of the outer membrane in this pathogen. The LPS inner core OS sugar composition in P. aeruginosa is identical among P. aeruginosa strains and it consists of two residues of 3-deoxy-d-manno-octulosonic acid (KdoI and KdoII) and two residues of L-glycero-d-manno-heptose (HepI and HepII). The high degree of phosphorylation of the inner core is essential for viability of P. aeruginosa.

P. aeruginosa Inner Core

The antibody recognizes the fully phosphorylated inner core OS of P. aeruginosa LPS.

5c-7-4

IgG2b kappa

None

Purified with protein G, stored in PBS pH 7.4 and lyophilized.

If reconstituted with deionized water in 100 µl: E: 1:1,000. Optimal dilution has to be determined by the user.

Research Use Only

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.