Robo4

The Robo (Roundabout) family of receptors is known for its guidance role during development. Studies have shown that one of the family members, Robo4, is specifically expressed in the vascular endothelium and may provide directional cues to migrating endothelial cells. Recent evidence shows that Robo4-deficient mice have increased angiogenesis, suggesting that Robo4 may play a role in restricting vascular sprouting.

Robo, roundabout guidance receptor 4, mRobo4-4CI5

Recombinant protein

The antibody recognizes Robo-4 protein.

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None

Lyophilized serum

If reconstituted with deionized water in 100 µl: WB 1:1000; FACS 1:300. Optimal dilution has to be determined by the user.

Research Use Only

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.