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Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)

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  • Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)
  • Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). 1: Ladder; 2: Wild type control Col0; 3: bmi1 mutant; 4: ring1 mutant. Same amount of proteins were loaded in each well. Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). 1: Ladder; 2: Wild type control Col0; 3: bmi1 mutant; 4: ring1 mutant. Same amount of proteins were loaded in each well.
  • Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). #1-2-3: Extraction pellets (negative control) - protein of interest should be located in the supernatant if extraction is efficient (#1 corresponds to extration pellet of #4, #2 of #5 and #3 of #6); #4: Total protein extraction (negative control) - Almost totally ineficient method for histone extraction; #5: nuclear protein extraction (Positive control - amount 1X); #6: nuclear protein extraction (Positive control - amount 2X). Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). #1-2-3: Extraction pellets (negative control) - protein of interest should be located in the supernatant if extraction is efficient (#1 corresponds to extration pellet of #4, #2 of #5 and #3 of #6); #4: Total protein extraction (negative control) - Almost totally ineficient method for histone extraction; #5: nuclear protein extraction (Positive control - amount 1X); #6: nuclear protein extraction (Positive control - amount 2X).
  • Western blot analysis of the ubiquitinated histone H2B antibody on whole cell extract from HeLa cells transfected with siRNA directed against the H2B E3 ubiquitin ligase (hBRE1) or LacZ. GAPDH antibody was used as loading control. Western blot analysis of the ubiquitinated histone H2B antibody on whole cell extract from HeLa cells transfected with siRNA directed against the H2B E3 ubiquitin ligase (hBRE1) or LacZ. GAPDH antibody was used as loading control.
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Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)

SKU:
MM-0029-P

USD 395.00 /100 µg

Clonality: 
Monoclonal
Host: Mouse
Reactivity: Human, Mouse, Drosophila, Arabidopsis
Application: WB, IHC, IF, ChIP

OVERVIEW

Target: 
Ubiquitinated H2B
Target background: 
It has been demonstrated that histone modifications are implicated in the regulation of transcription. Recent studies have revealed complex links between H2B ubiquitination and the control of transcription. Some studies suggest that H2B ubiquitination plays a role in transcriptional silencing whereas others suggest a role in transcriptional initiation and elongation. This antibody was raised against a branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B.
Target alias: 
Ubiquitinated Histone H2B, Ubiquitin Histone H2B, H2B, ub histone h2b, ub h2b
Immunogen: 
Branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B
Specificity: The antibody recognizes the K120 ubiquitination of the human histone H2B with no detectable cross-reactivity with either non-ubiquitinated H2B or ubiquitinated histone H2A.
Clone ID: 
NRO3
Isotype: 
IgG2a
Preservative: 
None
Format: 
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 50 µL: WB 2-3 µg/ml; ChIP 3-5 µg per sample; IHC: 1:200 – 1:500. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only
References: 
1.-Chen S - Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
2.-Dickson KA - The RING finger domain E3 ubiquitin ligases BRCA1 and the RNF20/RNF40 complex in global loss of the chromatin mark histone H2B monoubiquitination (...
3.-Lee S - Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases.
4.-Wu Y - Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination.
5.-Wijeweera A - Gonadotropin gene transcription is activated by menin-mediated effects on the chromatin.
6.-Rao SG - Invadolysin acts genetically via the SAGA complex to modulate chromosome structure.
7.-Ooga M - Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development.
8.-Bonizec M - The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B.
9.-Zou B - Monoubiquitination of histone 2B at the disease resistance gene locus regulates its expression and impacts immune responses in Arabidopsis.
10.-Ménard R - Histone H2B monoubiquitination is involved in the regulation of cutin and wax composition in Arabidopsis thaliana.
11.-Long L - The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination.
12.-Wu B - Ring Finger Protein 14 is a new regulator of TCF/β-catenin-mediated transcription and colon cancer cell survival.
13.-Armour SM - A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex.
14.-Nardini M - Sequence-specific transcription factor NF-Y displays histone-like DNA binding and H2B-like ubiquitination.
15.-Sin HS - RNF8 regulates active epigenetic modifications and escape gene activation from inactive sex chromosomes in post-meiotic spermatids.
16.-Santoro SW and Dulac C - The activity-dependent histone variant H2BE modulates the life span of olfactory neurons.
17.-Himanen K - Histone H2B monoubiquitination is required to reach maximal transcript levels of circadian clock genes in Arabidopsis.
18.-Mattiroli F - RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
19.-Bourbousse C - Histone H2B monoubiquitination facilitates the rapid modulation of gene expression during Arabidopsis photomorphogenesis.
20.-Urasaki Y - Coupling of glucose deprivation with impaired histone H2B monoubiquitination in tumors.
21.-Lee JS - Codependency of H2B monoubiquitination and nucleosome reassembly on Chd1.
22.-Jääskeläinen T - Histone H2B ubiquitin ligases RNF20 and RNF40 in androgen signaling and prostate cancer cell growth.
23.-Hahn MA - The tumor suppressor CDC73 interacts with the ring finger proteins RNF20 and RNF40 and is required for the maintenance of histone 2B monoubiquitina...
24.-Chipumuro E and Henriksen MA - The ubiquitin hydrolase USP22 contributes to 3'-end processing of JAK-STAT-inducible genes.
25.-Rytinki MM - Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans.
26.-Lang G - The tightly controlled deubiquitination activity of the human SAGA complex differentially modifies distinct gene regulatory elements.
27.-Vernimmen D - Polycomb eviction as a new distant enhancer function.
28.-Ma MK - Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.
29.-Taniue K - A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis.
30.-Shema E - Detection and characterization of ubiquitylated H2B in mammalian cells.
31.-Krichevsky A - Involvement of KDM1C histone demethylase-OTLD1 otubain-like histone deubiquitinase complexes in plant gene repression.
32.-Kim J and Roeder RG - Nucleosomal H2B ubiquitylation with purified factors.
33.-Roudier F - Integrative epigenomic mapping defines four main chromatin states in Arabidopsis.
34.-Gatta R - An acetylation-mono-ubiquitination switch on lysine 120 of H2B.
35.-Draker R - USP10 deubiquitylates the histone variant H2A.Z and both are required for androgen receptor-mediated gene activation.
36.-Gao Z - Resveratrol induces cellular senescence with attenuated mono-ubiquitination of histone H2B in glioma cells.
37.-Veiseth SV - The SUVR4 histone lysine methyltransferase binds ubiquitin and converts H3K9me1 to H3K9me3 on transposon chromatin in Arabidopsis.
38.-Gao Z and Xu CW - Glucose metabolism induces mono-ubiquitination of histone H2B in mammalian cells.
39.-Buro LJ - Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT...
40.-Gatta R and Mantovani R - Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters.
41.-Le May N - NER factors are recruited to active promoters and facilitate chromatin modification for transcription in the absence of exogenous genotoxic attack.
42.-Lilley CE - A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses.
43.-Lu LY - RNF8-dependent histone modifications regulate nucleosome removal during spermatogenesis.
44.-Mohan M - Linking H3K79 trimethylation to Wnt signaling through a novel Dot1-containing complex (DotCom).
45.-Stewart GS - The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.
46.-Schmitz RJ - Histone H2B deubiquitination is required for transcriptional activation of FLOWERING LOCUS C and for proper control of flowering in Arabidopsis.
47.-Sarkari F - EBNA1-mediated recruitment of a histone H2B deubiquitylating complex to the Epstein-Barr virus latent origin of DNA replication.
48.-Koues OI - The 19S proteasome positively regulates histone methylation at cytokine inducible genes.
49.-Buszczak M - Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny.
50.-Cao Y - Histone H2B monoubiquitination in the chromatin of FLOWERING LOCUS C regulates flowering time in Arabidopsis.
51.-Minsky N - Monoubiquitinated H2B is associated with the transcribed region of highly expressed genes in human cells.
52.-Shema E - The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression.
53.-US20130295584A1 - Histone protein ubiquitination as a cancer biomarker
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.